Parkinson's disease is the second most common debilitating neurological disorder worldwide, and unfortunately, there is still no definitive way to prevent it. Polyphenols have shown protective efficacy against various symptoms of Parkinson's disease.
However, there is still a lack of data on their effect on the underlying pathophysiological mechanisms of this disease. In this study, we evaluated the activity of a mix of polyphenols and micronutrients, named A5+ (SIRT500 Plus), in the N1E115 murine neuroblastoma cell line treated with 6-hydroxydopamine (6-OHDA), a well-established neurotoxic stimulus used to induce an in vitro Parkinson's disease model.
The study demonstrates that pre-treatment of these cells with A5+ leads to a significant reduction in inflammation, resulting in decreased pro-inflammatory cytokines (IFN-γ, IL-6, TNF-α, and CXCL1), reduced production of reactive oxygen species (ROS), activation of extracellular signal-regulated kinases (ERK1/2), and a decrease in apoptotic mechanisms with a corresponding increase in cell viability.
Interestingly, A5+ treatment promoted the differentiation of cells into dopaminergic neurons, as evidenced by enhanced expression of tyrosine hydroxylase, a well-established dopaminergic neuronal marker. Overall, these results demonstrate the synergistic and innovative efficacy of the A5+ mix against the cellular pathological processes of Parkinson's disease, although further studies are needed to elucidate the mechanisms underlying its beneficial effect.
